不同氮浓度条件下红色赤潮藻脂肪酸含量及组成特征     

刘云  陈田田  宋书群  李才文 《海洋与湖沼》2019,50(2):301-307

在不同氮浓度条件下对红色赤潮藻(Akashiwosanguinea)进行了一次性培养,研究了其生长过程中脂肪酸含量及组成变化。结果表明:红色赤潮藻脂肪酸含量及组成受初始氮浓度及生长阶段的影响。低氮处理组(0—25μmol/L)脂肪酸含量[(150.02±54.84)—(166.83±46.46)mg/gdw]显著高于其他处理组(P0.05),883μmol/L组最低,约为(86.70±5.66)mg/gdw;各培养组脂肪酸含量随着培养时间的延长而增加,稳定期时脂肪酸含量最高,指数生长前期含量最低。红色赤潮藻含28种脂肪酸,以C16:0、C18:1、C20:5ω3(EPA)和C22:6ω3(DHA)为主,且DHA相对含量较高,最高可占总脂肪酸含量的30.46%。随着初始氮浓度的增加,红色赤潮藻饱和脂肪酸(SFA)和单不饱和脂肪酸(MUFA)在总脂肪酸中所占比例下降,而多不饱和脂肪酸(PUFA)所占比例升高。在红色赤潮藻生长过程中,MUFA相对含量在稳定期时较高,而PUFA相对含量在指数生长前期较高。研究结果可为进一步揭示红色赤潮藻赤潮应对环境胁迫的生理响应机制提供依据。  相似文献   

Spatial and temporal variability of stable isotopes (δ18O and δ2H) in surface waters of arid,mountainous Central Asia     

Huawu Wu  Jinglu Wu  Kadyrbek Sakiev  Jinzhao Liu  Jing Li  Bin He  Ya Liu  Beibei Shen 《水文研究》2019,33(12):1658-1669

Characterization of spatial and temporal variability of stable isotopes (δ¹⁸O and δ²H) of surface waters is essential to interpret hydrological processes and establish modern isotope–elevation gradients across mountainous terrains. Here, we present stable isotope data for river waters across Kyrgyzstan. River water isotopes exhibit substantial spatial heterogeneity among different watersheds in Kyrgyzstan. Higher river water isotope values were found mainly in the Issyk‐Kul Lake watershed, whereas waters in the Son‐Kul Lake watershed display lower values. Results show a close δ¹⁸O–δ²H relation between river water and the local meteoric water line, implying that river water experiences little evaporative enrichment. River water from the high‐elevation regions (e.g., Naryn and Son‐Kul Lake watershed) had the most negative isotope values, implying that river water is dominated by snowmelt. Higher deuterium excess (average d = 13.9‰) in river water probably represents the isotopic signature of combined contributions from direct precipitation and glacier melt in stream discharge across Kyrgyzstan. A significant relationship between river water δ¹⁸O and elevation was observed with a vertical lapse rate of 0.13‰/100 m. These findings provide crucial information about hydrological processes across Kyrgyzstan and contribute to a better understanding of the paleoclimate/elevation reconstruction of this region.  相似文献   

An ensemble approach for the analysis of extreme rainfall under climate change in Naples (Italy)     

Roberta Padulano  Alfredo Reder  Guido Rianna 《水文研究》2019,33(14):2020-2036

In the present paper, an ensemble approach is proposed to estimate possible modifications caused by climate changes in the extreme precipitation regime, with the rain gauge Napoli Servizio Idrografico (Naples, Italy) chosen as test case. The proposed research, focused on the analysis of extremes on the basis of climate model simulations and rainfall observations, is structured in several consecutive steps. In the first step, all the dynamically downscaled EURO‐CORDEX simulations at about 12 km horizontal resolution are collected for the current period 1971–2000 and the future period 2071–2100, for the RCP4.5 and the RCP8.5 concentration scenarios. In the second step, the significance of climate change effects on extreme precipitation is statistically tested by comparing current and future simulated data and bias‐correction is performed by means of a novel approach based on a combination of simple delta change and quantile delta mapping, in compliance with the storm index method. In the third step, two different ensemble models are proposed, accounting for the variabilities given by the use of different climate models and for their hindcast performances. Finally, the ensemble models are used to build novel intensity–duration–frequency curves, and their effects on the early warning system thresholds for the area of interest are evaluated.  相似文献   

不同水位和竞争模式对典型湿地植物生态化学计量特征的影响     

李旭  于洁  李峰  胡聪  曾静  侯志勇  谢永宏  陈心胜 《湖泊科学》2019,31(6):1651-1661

以洞庭湖典型湿地植物辣蓼为目标植物,短尖苔草为邻近植物,通过控制实验研究不同水位(30、0和-30 cm)和竞争(无竞争、全部竞争、地上竞争和地下竞争)模式下目标植物生长及生态化学计量特征的变化.结果表明:水位处理显著影响不同竞争模式下的生物量积累,辣蓼生物量随水位增加显著降低;-30 cm水位无竞争模式下生物量最大,为10.84±1.52 g.在30 cm和0 cm水位梯度下,不同竞争模式下的生物量间无显著差异.但-30 cm水位下,地下竞争模式下的辣蓼生物量积累较全竞争模式和地上竞争模式下显著增多,说明非胁迫条件下,辣蓼和苔草的竞争以地上竞争为主.水位处理对辣蓼叶片、茎和根的氮、磷含量影响显著,30 cm水位下,叶片氮、磷含量显著高于其他水位下的含量.在-30cm水位下,叶片C∶N和C∶P显著高于其他水位下的比值,分别为48.08±3.85、590.3±43.4.相比于对照处理(无竞争),竞争作用下的辣蓼总氮含量降低,而C∶N值增加,N∶P值降低,这可能是因为竞争作用导致辣蓼对氮的吸收减少所致.  相似文献   

海州湾4种鱼类生长特征的空间异质性     

王琨  张崇良  王晶  任一平 《海洋学报》2019,41(12):62-70

传统的渔业资源评估均假设鱼类的生长参数是匀质的，然而近年来越来越多的研究表明海洋鱼类生长存在空间异质性。为探究海州湾鱼类生长参数的空间异质性现象，本研究分析了2013–2018年海州湾及其邻近海域方氏云鳚(Pholis fangi)、尖海龙(Syngnatus acus)、小黄鱼(Larimichthys polyactis)和赤鼻棱鳀(Thryssa kammalensis)的空间分布，使用电子体长频率分析方法结合Bootstrap重抽样方法估算了这4种鱼类的生长参数及其在深、浅水区域中的差异。结果显示，这4种鱼类生长参数均表现出一定的空间异质性，其中尖海龙和小黄鱼生长参数的空间异质性表现较为明显。这种差异可能是由于空间上的理化条件、群落结构以及物种本身洄游分布的差异而产生的。  相似文献   

九孔鲍MSTN基因cSNP多态性及与生长性状关联分析   总被引：1，自引：0，他引：1  

艾加林  栗志民  刘建勇  申玉春 《热带海洋学报》2019,38(2):78-85

九孔鲍(Haliotis diversicolor supertexta)是中国南方具有较高经济价值的养殖贝类。肌肉生长抑制素(Myostatin, MSTN)是转化生长因子β超家族(TGF-β)中参与动物肌肉生长的重要调控因子, 因此MSTN基因是动物遗传改良的重要候选基因。为探究九孔鲍MSTN基因的多态性及其生长相关性, 实验采用PCR产物直接测序方法对九孔鲍MSTN基因进行单核苷酸多态性(SNP)筛选, 对MSTN基因SNP与体质量、壳长、壳宽进行关联性分析, 运用实时荧光定量PCR (qRT-PCR)技术检测九孔鲍MSTN基因不同基因型的表达水平。结果显示: 在九孔鲍MSTN基因中共筛选出9个SNP位点。SNP位点与生长性状关联分析发现, 九孔鲍MSTN基因编码区SNP位点g909C>T发生C/T同义突变与九孔鲍体质量、壳长、壳宽显著相关。TT基因型九孔鲍的体质量显著高于CC基因型和TC基因型个体; TT基因型的壳长、壳宽显著高于CC基因型(p<0.05)。通过qRT-PCR检测显示, 在九孔鲍MSTN基因SNP位点g909C>T的3种基因型中, TC和CC基因型的基因表达量显著高于TT基因型(p<0.05)。研究结果表明MSTN基因SNP位点g909C>T可作为九孔鲍标记辅助选择育种重要候选标记。  相似文献   

The Cabo Frio upwelling overrides geographical patterns in the population dynamics of the shrimp Artemesia longinaris Spence Bate, 1888 (Decapoda: Penaeidae)     

Abner Carvalho‐Batista  Joo Alberto Farinelli Pantaleo  Antonio Leo Castilho  Rogrio Caetano da Costa 《Marine Ecology》2019,40(2)

Upwelling areas are among the most productive ecosystems on the planet, influencing the biology of marine organisms. This study investigated the population dynamics of the shrimp Artemesia longinaris in two regions in southeastern Brazil, one inside (Macaé—Rio de Janeiro State) and one outside (Ubatuba—State of São Paulo) the Cabo Frio upwelling area. The aim was to verify the influence of the upwelling phenomenon on the abundance, growth, longevity, size of sexual maturity, and reproductive period of the species. In total, 188,902 individuals were captured at Macaé and 3,461 at Ubatuba. Individuals captured at Macaé showed larger maximum size, higher longevity, and slower growth rate, besides reaching sexual maturity at larger sizes than at Ubatuba. Continuous reproduction was observed in both regions, with juvenile recruitment peaks in spring and summer. Local conditions observed at Macaé were influenced by the Cabo Frio upwelling zone, characterized by productive and cooler waters that are around 20°C during most of the year. The upwelling phenomenon is probably the main factor influencing the population parameters studied here, changing the geographic patterns previously observed for the variation of these parameters in A. longinaris.  相似文献   

氨氮急性攻毒对水产经济动物棘胸蛙(Paa spinosa)蝌蚪死亡率、排氨率、耗氧率及窒息点的影响     

牛春格  杨程  申屠琰  郑晓静  王志铮 《海洋与湖沼》2019,50(1):188-196

以体长(1.932±0.204)cm、体质量(1.386±0.055)g的棘胸蛙蝌蚪为实验动物,在水温(24±0.2)℃、DO(7.30±0.01)mg/L、pH 7.30±0.01条件下,采用静水停食法开展了氨氮对蝌蚪的急性攻毒实验,并以此为基础,测定了不同氨氮质量浓度胁迫下棘胸蛙蝌蚪的排氨率、耗氧率、氧氮比及窒息点。结果表明:(1)蝌蚪对氨氮急性攻毒具明显的运动避毒行为,濒死个体的背部皮肤和肝脏均具明显的色变症状;(2)氨氮对蝌蚪的急性致死率具明显的剂量—时间效应,24h、48h、72h、96h LC50依次为177.5、151.7、148.6和146.8mg/L,毒性时段蓄积程度系数呈持续下降趋势,24—48h、48—72h、72—96h的时段MAC值分别为84.04%、10.1%和5.86%;(3)蝌蚪夜均、昼均、日均及时段排氨率随氨氮质量浓度增加均呈阶梯式下降趋势,其中时段排氨率与对照组均无显著差异的为9.80mg/L实验组,夜均、昼均、日均及时段排氨率与对照组均无显著差异的仅为2.45mg/L实验组(P0.05);(4)氨氮对蝌蚪呼吸耗氧具低毒兴奋效应,其夜均、昼均和日均耗氧率随氨氮质量浓度增加均呈先升后降趋势,峰值氨氮质量浓度范围均为4.90—7.35mg/L(P0.05),与对照组均无显著差异的仅为14.70mg/L实验组(P0.05),窒息点含氧量随氨氮质量浓度增加呈先降后升趋势,谷底出现于4.90mg/L实验组(P0.05),与对照组无显著差异的仅为9.80mg/L实验组(P0.05);(5)实验期间,蝌蚪氧氮比(O:N)波动于18.87—25.34之间,昼均和日均氧氮比(O:N)随氨氮质量浓度增加均依次呈"上升—稳定—下降—再稳定"之趋势,两者的峰值氨氮质量浓度范围分别为4.90—14.70mg/L和4.90—9.80mg/L,夜均氧氮比(O:N)呈先升后降趋势,峰值出现于7.35mg/L实验组(P0.05)。  相似文献   

Analysis of the extreme wave elevation due to second-order diffraction around a vertical cylinder     

《Applied Ocean Research》2019

Statistical analysis of nonlinear random waves is important in coastal and ocean engineering. One approach for modeling nonlinear waves is second-order random wave theory, which involves sum- and difference-frequency interactions between wave components. The probability distribution of the non-Gaussian surface elevation can be solved using a technique developed by Kac and Siegert [21]. The wave field can be significantly modified by wave diffraction due to a structure, and the nonlinear diffracted wave elevation can be of interest in certain applications, such as the airgap prediction for an offshore structure. This paper investigates the wave statistics due to second-order diffraction, motivated by the scarcity of prior research. The crossing rate approach is used to evaluate the extreme wave elevation over a specified duration. The application is a bottom-supported cylindrical structure, for which semi-analytical solutions for the second-order transfer functions are available. A new efficient statistical method is developed to allow the distribution of the diffracted wave elevation to be obtained exactly, accounting for the statistical dependency between the linear, sum-frequency and difference-frequency components. Moreover, refinements are proposed to improve the efficiency for computing the free surface integral. The case study yields insights into the problem. In particular, the second-order nonlinearity is found to significantly amplify the extreme wave elevation, especially in the upstream region; conversely, the extreme elevation at an oblique location downstream is attenuated due to sheltering effects. The statistical dependency between the linear and sum-frequency components is also shown to be important for the extreme wave statistics.  相似文献   

LATE QUATERNARY ACTIVITY OF THE SOUTHEASTERN SECTION OF ZHONGDIAN-DAJU FAULT          下载免费PDF全文

LI Guang-tao  SU Gang  CHENG Li  LI Feng  WU Hao 《地震地质》2019,41(3):545-560

The southeast section of Zhongdian-Daju Fault is located in the northern part of Haba and Yulong Snow Mountain, belonging to the southwestern boundary of the secondary block in northwestern Sichuan, an important boundary fault striking 310°~320° on the whole. The nature of the fault, the age of its activity and the slip rate are of great significance for the analysis of the secondary block movement in the northwestern Sichuan and the intersection relationship with the eastern piedmont fault of Yulong Mountains. Based on the 1 ︰ 5 million-scale active fault geological mapping, this paper studies in detail the stratigraphic landform, scarp landform, surface rupture, typical fault profile and river terrace along the fault. Based on the research results, we divide the southeastern section of Zhongdian-Daju Fault into two sub-segments, the Majiacun-Daju sub-segment and the Daju-Dadong sub-segment, according to the geometric structure, fault landforms and fault activity. (1)Fault scarp:In the Majiacun-Daju sub-segment, the fault parallelly controls the two sides of the Haba fault depression. It cuts the late Pleistocene moraine deposits, forming a fault scarp of about 4.5km long and(14±2)m high. The continuity of the scarp is very good, and it is also very obvious in the remote sensing image. In the Daju-Dadong sub-segment, a scarp with a height of about 2m is formed, and an optical luminescence dating sample is collected from the upper part of the gravel layer on the second-order terrace to obtain an age of(22±2.2)ka. (2)Horizontal dislocation:In the Majiacun-Daju sub-segment, through the analysis of the development of outwash fans in the area and the measurement and induction of the gully dislocations, it is considered that there are at least three stages of outwash fans developed in the area and there may be four phases of faulting. That is, the earliest-stage outwash fan and gully are horizontally dislocated about 1km; the second-stage outwash fan and gully are horizontally dislocated about 47m, and the vertical dislocation is about(14±2)m; the gully in the third stage outwash fan is horizontally dislocated twice, the first dislocation formed a beheaded gully with a dislocation of 22m, and the second formed a beheaded gully with a dislocation of 8.5m. It is further proved that the fault has strong activity since the Holocene in the Majiacun to Daju area. In the Daju-Dadong sub-segment, there are no obvious horizontal dislocations in the alluvial deposits since the Holocene. Only 3~4 gullies are found to be offset right-laterally in the ridges east of Wenhe Village, with the maximum dislocation of 210m, which may be the older phase dislocation. (3)Surface rupture:In the northwest direction of Dabazi Village on the T3 terrace in the basin between Majiacun and Daju, an earthquake surface rupture zone is found, extending in the NW direction. The rupture zone left clear traces on the about 1m-thick, hard T₃ terrace surface formed by calcification of sand gravels, and the overburden either upwarps and bulges, or ruptures, generates ground fissures, or forms small pull-apart "depressions" locally. However, the rupture zone is not large in size, about 350m long, 60m wide at the widest point, and 0.3~1.5m high. It is partially en-echelon or obliquely arranged, dominated by compressive ruptures. Through observation, the possibility of artificial transformation is ruled out for these upwarping bulges, ruptures or ground fissures. The fault section is found in the southeast direction of the rupture zone. The slickensides at the section show that the fault is dominated by right-lateral strike-slip with a small amount of thrust. In the eastern sub-segment, only intermittently distributed surface ruptures are found in the northern part of the village, and the scale is small. In summary, through the field geological survey, it is found that the Majiacun-Daju sub-segment is a Holocene active segment. Though the Daju-Dadong sub-segment also offset the late Pleistocene to Holocene strata, it is considered that its Holocene activity is weak in terms of either the dislocation amount or the slip rate of this segment. By analyzing the geological and geomorphological evidences, such as fault scarps, horizontal dislocation and surface ruptures along the fault, it is considered that the Majiacun-Daju sub-segment is a right-lateral strike-slip fault with a normal faulting component, and its vertical slip rate since the late Pleistocene is(0.4~0.8)mm/a, the horizontal slip rate is 1.5~2.4mm/a. The Daju-Dadong sub-segment is dominated by right-lateral strike-slip with a normal faulting component, and its vertical slip rate since the late Late Cenozoic is 0.1mm/a. The formation of the NW-trending surface rupture zone found in the Daju Basin is very young, where there are only two major earthquakes, namely, the M_S6.4 1966 Zhongdian earthquake and the 1996 Lijiang M_S7.0 earthquake, and both earthquakes produced NW-oriented surface rupture zones. Therefore, it cannot be ruled out that the rupture zone is a product of the 1966 Zhongdian M_S6.4 earthquake or the 1996 Lijiang M_S7.0 earthquake.  相似文献